Microtubule Capture: IQGAP and CLIP-170 Expand the Repertoire
نویسندگان
چکیده
منابع مشابه
Microtubule Capture: IQGAP and CLIP-170 Expand the Repertoire
Rho GTPases regulate microtubule capture near the cell cortex to polarize cells. What is surprising is the repertoire of interactions between proteins at the ends of microtubules and their cortical targets. The microtubule tip protein CLIP-170 has now been found to interact with the Cdc42/Rac effector IQGAP and mediate transient capture of microtubules.
متن کاملCLIP-170 facilitates the formation of kinetochore-microtubule attachments.
CLIP-170 is a microtubule 'plus end tracking' protein involved in several microtubule-dependent processes in interphase. At the onset of mitosis, CLIP-170 localizes to kinetochores, but at metaphase, it is no longer detectable at kinetochores. Although RNA interference (RNAi) experiments have suggested an essential role for CLIP-170 during mitosis, the molecular function of CLIP-170 in mitosis ...
متن کاملRac1 and Cdc42 Capture Microtubules through IQGAP1 and CLIP-170
Linkage of microtubules to special cortical regions is essential for cell polarization. CLIP-170 binds to the growing ends of microtubules and plays pivotal roles in orientation. We have found that IQGAP1, an effector of Rac1 and Cdc42, interacts with CLIP-170. In Vero fibroblasts, IQGAP1 localizes at the polarized leading edge. Expression of carboxy-terminal fragment of IQGAP1, which includes ...
متن کاملMicrotubule plus-end tracking by CLIP-170 requires EB1.
Microtubules are polarized polymers that exhibit dynamic instability, with alternating phases of elongation and shortening, particularly at the more dynamic plus-end. Microtubule plus-end tracking proteins (+TIPs) localize to and track with growing microtubule plus-ends in the cell. +TIPs regulate microtubule dynamics and mediate interactions with other cellular components. The molecular mechan...
متن کاملCLIP-170 Highlights Growing Microtubule Ends In Vivo
A chimera with the green fluorescent protein (GFP) has been constructed to visualize the dynamic properties of the endosome-microtubule linker protein CLIP170 (GFP-CLIP170). GFP-CLIP170 binds in stretches along a subset of microtubule ends. These fluorescent stretches appear to move with the growing tips of microtubules at 0.15-0.4 microm/s, comparable to microtubule elongation in vivo. Analysi...
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ژورنال
عنوان ژورنال: Current Biology
سال: 2002
ISSN: 0960-9822
DOI: 10.1016/s0960-9822(02)01156-9